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hek293 t crl 3216 cells  (ATCC)


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    Structured Review

    ATCC hek293 t crl 3216 cells
    Hek293 T Crl 3216 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek293+t+cells/293T/pm42320680-165-0-6
    Average 99 stars, based on 37978 article reviews
    hek293 t crl 3216 cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: The LOK C-Terminus is an IBAR-like Domain that Facilitates Membrane Binding and Ezrin Colocalization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Isolation:

    Article Title: The LOK C-Terminus is an IBAR-like Domain that Facilitates Membrane Binding and Ezrin Colocalization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    other:

    Article Title: Chemically-stabilized allosteric modulators of leucine-rich repeat kinase 2 (LRRK2)
    Article Snippet: All synthesis reagents and solvents were purchased from Fisher, Sigma-Aldrich, or Acros.

    Cell Culture:

    Article Title: Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndrome.
    Article Snippet: .. Briefly, HEK293-T cells (CRL-3216, ATCC; tested for mycoplasma and STR profiled by ATCC) were cultured in 12-well plates seeded at 3.5 × 105 cells per well and maintained in DMEM (11995065, Gibco) with 10% fetal bovine serum (FBS). .. After 24-h, when cells were about 50–80% confluent, cells were co-transfected with 400 ng of pAAVCas9-gRNA or pAAV-ZFN plasmid, 400 ng of AAV2/1 serotype plasmid (a gift from James M. Wilson; 112862, Addgene, Watertown, MA, USA), and 800 ng of pAdDELTAF6 helper plasmid (a gift from James M. Wilson; 112867, Addgene) using FuGENE 6 (E2691, Promega).

    Article Title: Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndrome
    Article Snippet: .. Briefly, HEK293-T cells (CRL-3216, ATCC; tested for mycoplasma and STR profiled by ATCC) were cultured in 12-well plates seeded at 3.5 × 10 5 cells per well and maintained in DMEM (11995065, Gibco) with 10% fetal bovine serum (FBS). .. After 24-h, when cells were about 50–80% confluent, cells were co-transfected with 400 ng of pAAV-Cas9-gRNA or pAAV-ZFN plasmid, 400 ng of AAV2/1 serotype plasmid (a gift from James M. Wilson; 112862, Addgene, Watertown, MA, USA), and 800 ng of pAdDELTAF6 helper plasmid (a gift from James M. Wilson; 112867, Addgene) using FuGENE 6 (E2691, Promega).

    Clone Assay:

    Article Title: Glypican-2-binding moieties, chimeric antigen receptors and uses thereof
    Article Snippet: .. HEK293T clones that stably express human GPC2, Cynomolgus monkey GPC2, Pteropus vampyrus GPC2 were prepared by transfecting HEK293 T cells (ATCC, #CRL-1573) with plvx-EF1α-puro plasmids comprising sequences of human GPC2 (NM 152742.3), Cynomolgus monkey (Macaca fascicularis) GPC2 (NW 005092963.1) or Pteropus vampyrus GPC2 ORF (NW_011888974.1). .. Phage supernatant was added to the GPC2-expressing HEK293T stable cells and SH-SY5Y tumor cell (ATCC, #CRL-2266), the residual phage that bound on cell surface was detected by M13 Bacteriaphage Antibody (Sino biological, #11973-MM05T-H).

    Stable Transfection:

    Article Title: Glypican-2-binding moieties, chimeric antigen receptors and uses thereof
    Article Snippet: .. HEK293T clones that stably express human GPC2, Cynomolgus monkey GPC2, Pteropus vampyrus GPC2 were prepared by transfecting HEK293 T cells (ATCC, #CRL-1573) with plvx-EF1α-puro plasmids comprising sequences of human GPC2 (NM 152742.3), Cynomolgus monkey (Macaca fascicularis) GPC2 (NW 005092963.1) or Pteropus vampyrus GPC2 ORF (NW_011888974.1). .. Phage supernatant was added to the GPC2-expressing HEK293T stable cells and SH-SY5Y tumor cell (ATCC, #CRL-2266), the residual phage that bound on cell surface was detected by M13 Bacteriaphage Antibody (Sino biological, #11973-MM05T-H).

    Plasmid Preparation:

    Article Title: Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia‐inducible factor 1‐alpha/ubiquitin protein ligase E3 component N‐recognin 5 axis
    Article Snippet: All plasmids were synthesized by Tsingke Biotechnology (Beijing, China). .. HEK293 T cells (CRL‐3216, ATCC, USA) were seeded in 24‐well plates at a density of 5 × 10 4 cells per well and co‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. ..

    Luciferase:

    Article Title: Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia‐inducible factor 1‐alpha/ubiquitin protein ligase E3 component N‐recognin 5 axis
    Article Snippet: All plasmids were synthesized by Tsingke Biotechnology (Beijing, China). .. HEK293 T cells (CRL‐3216, ATCC, USA) were seeded in 24‐well plates at a density of 5 × 10 4 cells per well and co‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. ..

    Control:

    Article Title: Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia‐inducible factor 1‐alpha/ubiquitin protein ligase E3 component N‐recognin 5 axis
    Article Snippet: All plasmids were synthesized by Tsingke Biotechnology (Beijing, China). .. HEK293 T cells (CRL‐3216, ATCC, USA) were seeded in 24‐well plates at a density of 5 × 10 4 cells per well and co‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. ..



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